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Equipment

Flow Cytometers (analysers)

Analysers are available for users 24/7. Please contact for details.

Cytoflex LX – has 4 lasers: 405, 488, 561 and 640 nm. It has 16 fluorescent channels available and 96wp reader.

BD Fortessa X20 - has 5 lasers: 355 (UV), 405, 488, 561 and 640 nm. It has 18 fluorescent channels available. Cytometer is equipped with HTS (High Throughput System – 96wp reader).

Cell Sorters

Sorts are performed by authorised operators only.

BD Aria III - has 4 lasers: 405, 488, 561 and 640 nm. It has 16 fluorescent channels available and is capable of single cell sorts into 96 well plates as well as bulk sorting. A choice of nozzle sizes are available: 70, 85, 100, 130 um.

MoFlo Astrios - has 4 lasers: 405, 488, 561 and 640 nm. It has 16 fluorescent channels available and is capable of single cell sorts into 96 well plates as well as bulk sorting. A choice of nozzle sizes are available: 70, 100, 150, 200 um.

Imaging Flow Cytometer

Amnis Imagestream MKII imaging flow cytometer – Best described as a high throughput microscope, this machine enables users to take a high resolution picture (optical magnification of 20x, 40x or 60x) of every single event and overlay flow data with microscopy picture. Cytometer is equipped with 4 lasers: 405, 488, 561 and 638 nm offering 12 fluorescent channels.

Bacteria sorter:

There is a BioRad eS3 available at Biochemistry department, equipped with 2 lasers - 488nm and 561 nm. Machine is capable of sorting 2 population at the time and can read up to 4 fluorochromes simultaneously.

For charging, usage policy and availability please contact Matteo Ferla ( matteo.ferla@bioch.ox.ac.uk )